Comparative analysis of structural properties of Portunid Crab Hemocyanin.
نویسندگان
چکیده
In order to explore the hemocyanin adaptative potential and evolutive dynamics, we have analyzed the structural properties of this oxygen-carrier protein, in some species of portunid Crabs, (Brachyura, Portunida). We have compared the intra- and interspecific subunits patterns, in native and denaturant conditions, to estimate the phenetic relationships and the different stabilities of the protein.
منابع مشابه
Functional conversion of hemocyanin to phenoloxidase by horseshoe crab antimicrobial peptides.
Arthropod hemocyanins and phenoloxidases serve different physiological functions as oxygen transporters and enzymes involved in defense reactions, respectively. However, they are equipped with a structurally similar oxygen-binding center. We have shown that the clotting enzyme of the horseshoe crab, Tachypleus tridentatus, functionally converts hemocyanin to phenoloxidase by forming a complex w...
متن کاملHemocyanin of the Horseshoe Crab, Limzdus polyphemus
The high molecular weight hemocyanin found in the hemolymph of the horseshoe crab, Limulus polyphemus, is composed of at least eight different kinds of subunits. Ion exchange chromatography at high pH in the presence of EDTA yields five major zones, hemocyanins I to V, three of which are electrophoretically heterogeneous. The subunits have similar molecular weights, 65,000 to 70,000, and their ...
متن کاملPortunid crab predation on juvenile hard clams: effects of substrate type and prey density
Studies on predat~on are often based solely on the measurement of predator consumption rates, providing little information on underlying behavioral mechan~sms. In this study, behav~oral observations were used In conjunchon with consumption rate analyses to investigate the predatory interactions between 2 brachyuran crabs, Ovalipes ocellatus and Calllnectes sapidus, and juvenile hard clams Aderc...
متن کاملHemocyanins and the immune response: defense against the dark arts.
The innate immune response is a conserved trait shared by invertebrates and vertebrates. In crustaceans, circulating hemocytes play significant roles in the immune response, including the release of prophenoloxidases. Activated phenoloxidase (tyrosinase) participates in encapsulation and melanization of foreign organisms as well as sclerotization of the new exoskeleton after wound-repair or mol...
متن کاملOntogeny of hemocyanin function in the dungeness crab cancer magister: hemolymph modulation of hemocyanin oxygen-binding
The in vivo oxygen-binding characteristics of Cancer magister whole hemolymph were compared across developmental stages with those of purified hemocyanin. When the 25S hemocyanins from first-instar juvenile and adult C. magister were dialyzed against first-instar juvenile saline, the P50 values at pH 7.8 differed by 54 %: 2.16 kPa for the adult and 4.68 kPa for the first-instar juvenile. Since ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Micron
دوره 35 1-2 شماره
صفحات -
تاریخ انتشار 2004